Rescue of synthetic analogs of genomic RNA and replicative-intermediate RNA of human parainfluenza virus type 3
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چکیده
منابع مشابه
Rescue of synthetic genomic RNA analogs of rabies virus by plasmid-encoded proteins.
Proteins entirely expressed from cDNA were used to rescue synthetic RNA genome analogs into infectious defective particles of rabies virus (RV). Synthetic negative-stranded RNAs containing 3'- and 5'-terminal RV sequences and transcriptional signal sequences were transcribed from plasmids transfected into cells expressing T7 RNA polymerase from recombinant vaccinia virus. After simultaneous exp...
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چکیده ندارد.
Dengue virus replicative intermediate RNA detection by reverse transcription-PCR.
Dengue virus replication involves synthesis of a replicative intermediate RNA (RI-RNA), whose presence reveals an actual infection. We report on a simple and rapid reverse transcription-PCR for the detection of viral RI-RNA in infected cells. The product is demonstrated at 20 min postinfection. This method is useful for the study of virus-cell tropism.
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Mouse hepatitis virus A59 codes for seven mRNAs in infected cells. These mRNAs are transcribed from a minus (-) strand template of genome length and contain a leader RNA at their 5' ends. To further elucidate the mechanism of coronavirus transcription, we examined the structure of mouse hepatitis virus replicative intermediates (RIs) isolated by 2 M NaCl precipitation and Sepharose 2-B column c...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1993
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.67.5.2772-2778.1993